Detection of oligonucleotides by external injection into an ion trap storage/reflection time-of-flight device.
Identifieur interne : 002A89 ( Ncbi/Merge ); précédent : 002A88; suivant : 002A90Detection of oligonucleotides by external injection into an ion trap storage/reflection time-of-flight device.
Auteurs : L. He [États-Unis] ; Y H Liu ; Y. Zhu ; D M LubmanSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 1997.
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Abstract
The detection of oligonucleotides has been studied using external injection of Matrix-assisted laser desorption/ionization (MALDI) produced ions into a quadrupole ion trap storage device. The ions stored in the trap are then detected by pulsed DC injection into a reflection time-of-flight mass spectrometer. It is shown that by using a low acceleration voltage from the probe tip and external injection into the trap that 3-mers to 9-mers can be stored for 20-50 ms and detected as intact ions. In comparison, direct MALDI inside the trap resulted in extensive fragmentation as a function of storage time. Using the external injection with a He buffer gas in the trap yields a resolution of 250-400 in the ion trap storage/reflectron time-of-flight mass spectrometer. The resolution can be further enhanced to 400-800 using a bipolar extraction method. In addition, the trapping efficiency for the injection of externally produced ions into the trap can be enhanced using a dynamic trapping method. The MALDI mass spectra produced by this methodology result in excellent signal-to-noise ratio due to the capabilities of the trap for eliminating low mass matrix background from the spectra.
DOI: 10.1002/(SICI)1097-0231(19970830)11:13<1440::AID-RCM25>3.0.CO;2-9
PubMed: 9299762
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Zhu</name></author><author><name sortKey="Lubman, D M" sort="Lubman, D M" uniqKey="Lubman D" first="D M" last="Lubman">D M Lubman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9299762</idno><idno type="pmid">9299762</idno><idno type="doi">10.1002/(SICI)1097-0231(19970830)11:13<1440::AID-RCM25>3.0.CO;2-9</idno><idno type="wicri:Area/PubMed/Corpus">002741</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002741</idno><idno type="wicri:Area/PubMed/Curation">002741</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002741</idno><idno type="wicri:Area/PubMed/Checkpoint">002592</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002592</idno><idno type="wicri:Area/Ncbi/Merge">002A89</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of oligonucleotides by external injection into an ion trap storage/reflection time-of-flight device.</title><author><name sortKey="He, L" sort="He, L" uniqKey="He L" first="L" last="He">L. He</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Michigan, Ann Arbor 48109-1055</wicri:regionArea><wicri:noRegion>Ann Arbor 48109-1055</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Y H" sort="Liu, Y H" uniqKey="Liu Y" first="Y H" last="Liu">Y H Liu</name></author><author><name sortKey="Zhu, Y" sort="Zhu, Y" uniqKey="Zhu Y" first="Y" last="Zhu">Y. Zhu</name></author><author><name sortKey="Lubman, D M" sort="Lubman, D M" uniqKey="Lubman D" first="D M" last="Lubman">D M Lubman</name></author></analytic><series><title level="j">Rapid communications in mass spectrometry : RCM</title><idno type="ISSN">0951-4198</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electronic Data Processing</term><term>Gas Chromatography-Mass Spectrometry</term><term>Oligonucleotides (analysis)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie gazeuse-spectrométrie de masse</term><term>Oligonucléotides (analyse)</term><term>Spectrométrie de masse MALDI</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Oligonucléotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electronic Data Processing</term><term>Gas Chromatography-Mass Spectrometry</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie gazeuse-spectrométrie de masse</term><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The detection of oligonucleotides has been studied using external injection of Matrix-assisted laser desorption/ionization (MALDI) produced ions into a quadrupole ion trap storage device. The ions stored in the trap are then detected by pulsed DC injection into a reflection time-of-flight mass spectrometer. It is shown that by using a low acceleration voltage from the probe tip and external injection into the trap that 3-mers to 9-mers can be stored for 20-50 ms and detected as intact ions. In comparison, direct MALDI inside the trap resulted in extensive fragmentation as a function of storage time. Using the external injection with a He buffer gas in the trap yields a resolution of 250-400 in the ion trap storage/reflectron time-of-flight mass spectrometer. The resolution can be further enhanced to 400-800 using a bipolar extraction method. In addition, the trapping efficiency for the injection of externally produced ions into the trap can be enhanced using a dynamic trapping method. The MALDI mass spectra produced by this methodology result in excellent signal-to-noise ratio due to the capabilities of the trap for eliminating low mass matrix background from the spectra.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9299762</PMID><DateCompleted><Year>1997</Year><Month>10</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0951-4198</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>13</Issue><PubDate><Year>1997</Year></PubDate></JournalIssue><Title>Rapid communications in mass spectrometry : RCM</Title><ISOAbbreviation>Rapid Commun. Mass Spectrom.</ISOAbbreviation></Journal><ArticleTitle>Detection of oligonucleotides by external injection into an ion trap storage/reflection time-of-flight device.</ArticleTitle><Pagination><MedlinePgn>1440-8</MedlinePgn></Pagination><Abstract><AbstractText>The detection of oligonucleotides has been studied using external injection of Matrix-assisted laser desorption/ionization (MALDI) produced ions into a quadrupole ion trap storage device. The ions stored in the trap are then detected by pulsed DC injection into a reflection time-of-flight mass spectrometer. It is shown that by using a low acceleration voltage from the probe tip and external injection into the trap that 3-mers to 9-mers can be stored for 20-50 ms and detected as intact ions. In comparison, direct MALDI inside the trap resulted in extensive fragmentation as a function of storage time. Using the external injection with a He buffer gas in the trap yields a resolution of 250-400 in the ion trap storage/reflectron time-of-flight mass spectrometer. The resolution can be further enhanced to 400-800 using a bipolar extraction method. In addition, the trapping efficiency for the injection of externally produced ions into the trap can be enhanced using a dynamic trapping method. The MALDI mass spectra produced by this methodology result in excellent signal-to-noise ratio due to the capabilities of the trap for eliminating low mass matrix background from the spectra.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Y H</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Lubman</LastName><ForeName>D M</ForeName><Initials>DM</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1R01 HG00685-03</GrantID><Acronym>HG</Acronym><Agency>NHGRI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Rapid Commun Mass Spectrom</MedlineTA><NlmUniqueID>8802365</NlmUniqueID><ISSNLinking>0951-4198</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009841">Oligonucleotides</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001330" MajorTopicYN="N">Electronic Data Processing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008401" MajorTopicYN="N">Gas Chromatography-Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009841" MajorTopicYN="N">Oligonucleotides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>9</Month><Day>23</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9299762</ArticleId><ArticleId IdType="pii">10.1002/(SICI)1097-0231(19970830)11:13<1440::AID-RCM25>3.0.CO;2-9</ArticleId><ArticleId IdType="doi">10.1002/(SICI)1097-0231(19970830)11:13<1440::AID-RCM25>3.0.CO;2-9</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Liu, Y H" sort="Liu, Y H" uniqKey="Liu Y" first="Y H" last="Liu">Y H Liu</name><name sortKey="Lubman, D M" sort="Lubman, D M" uniqKey="Lubman D" first="D M" last="Lubman">D M Lubman</name><name sortKey="Zhu, Y" sort="Zhu, Y" uniqKey="Zhu Y" first="Y" last="Zhu">Y. Zhu</name></noCountry><country name="États-Unis"><noRegion><name sortKey="He, L" sort="He, L" uniqKey="He L" first="L" last="He">L. He</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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